EXTRACTION OF CHITOSAN FROM DIED HONEY BEE APIS MELLIFERA

Authors

Gulnora Akmalovna Ixtiyarova, Professor head of Deparment of General chemistry, Tashkent state technical university, Address: 2 Universitetskaya st., 100095, Tashkent city, Republic of Uzbekistan Tel: (+99891) 745-76-04, E-mail: gulnora74@mail.ru;Follow
Dilshoda Azamovna Hazratova, Asistent deparment Chemistry, Bukhara state university, Address: 11 Muxammad Ikbol st., 705018, Bukhara city, Republic of Uzbekistan Tel: (+99891) 440-82-83, E-mail: dilshoda.hazratova@mail.ru;Follow
Bobur Nosir o’g’li Umarov, Deparment of General chemistry, Tashkent state technical university, Address: 2 Universitetskaya st., 100095, Tashkent city, Republic of Uzbekistan
Oksana Muratbayevna Seytnazarova, Deparment of General chemistry, Tashkent state technical university, Address: 2 Universitetskaya st., 100095, Tashkent city, Republic of UzbekistanFollow

Abstract

This article presents the results of obtaining biopolymers of chitin and chitosan from a new promising source- died honey bee apis mellifera. To obtain chitin, at the first stage, the protein (deproteination) and mineral (demineralization) constituents of subpestilence, i.e. put them in soluble state and removed. Demineralization was carried out with 2 M hudrochloric acid for 5 hours at room temperature. The process of deproteination is carried out by processing the crushed raw materials with 1 N sodium hudroxide solution for 1 hour at a temperature of 80⁰С. Each process was accompanied by washing the raw materials to a natural wash water reaction (pH=7). At the next stage, chitin deacetylation under the influence of a 35% aqueous NaOH solution at a temperature of 85⁰С for 4 hours produced chitosan, which is a high molecular weight glucosamine polymer, and dried at 50-55⁰С. The resulting mass was decolorized with a 3% H₂O₂ solution and washed ethanol.

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References

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Recommended Citation

Ixtiyarova, Gulnora Akmalovna; Hazratova, Dilshoda Azamovna; Umarov, Bobur Nosir o’g’li; and Seytnazarova, Oksana Muratbayevna (2020) "EXTRACTION OF CHITOSAN FROM DIED HONEY BEE APIS MELLIFERA," Chemical Technology, Control and Management: Vol. 2020: Iss. 2, Article 3.
DOI: https://doi.org/10.34920/2020.2.15-20